Few studies have reported obtaining grass carp resistant to hemorrhagic disease via gene editing in commercial fish. Here. we demonstrate that the expression and activity of grass carp PI4KB (gcPI4KB) are vital for GCRV-I and GCRV-II replication. Given the obvious cytopathic effect (CPE) in the present available cell lines is only caused by GCRV-I. https://danddcollectiblers.shop/product-category/bird-baths/